Composite

Part:BBa_K5422007:Design

Designed by: HOAREAU Romain   Group: iGEM24_Aix-Marseille   (2024-09-25)


Inducible Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 847
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2061


Design Notes

During the design of this sequence, we optimized the codons for efficient expression in *E. coli* and ensured compatibility with BioBrick and Type IIS assembly standards. Additionally, we made adjustments to prevent secondary structures that could affect transcription and translation efficiency, and to facilitate the synthesis process by IDT. Importantly, these modifications involved synonymous codon changes, ensuring no alteration in the amino acid sequence.


Source

The DNA sequence for this part was synthesized by Integrated DNA Technologies (IDT) based on a custom design tailored for our project specifications.

References